FACS Aria IIu

Aria in Cabinet - 2013 (2)The BD FACS Aria IIu cell sorter is a highly flexible flow cytometry platform that enables the identification and collection of cells from complex mixtures (spleen, bone marrow, blood, mixed cell cultures) at several pressures and speeds depending sample requirements.

The instrument has 11 fluorescent detectors, 5 of which are dedicated to fluorophores excited at 488 nm (blue), plus three each at red (633 nm) and violet (405 nm) wavelengths.

The instrument is housed inside a class II, A2 biosafety cabinet for sorting of BSL-2 samples such as lentiviral infected cell lines, bacteria and infected/non-infected human samples.

When preparing your cells, please refer to our page on Sample Preparation for Sorting and concentrate your samples according to the guidelines below.

 

Instrument Specifications
Sort Set-ups
Nozzle (µM) 70 85 100
Pressure (PSI) 70

(high)

35

(medium)

30

(medium)

20

(low)

Cell Type Splenocytes

Thymocytes

PBMC

whole blood

bacteria

yeast

bone marrow

B/T cell lines

Embryonic Stem Cells

Transduced/Transfected spleen, thymus bone marrow (delicate) cells

 

Embryonic stem cells

Trophoblast stem cells

Cultured primary cells

Endothelial cells

All adherent cell lines

Newly transfected cells

Large delicate primary cells*

Cell Concentration** 40 million/mL 10 – 15 million/mL 8 million/mL 3-5 million/mL

(Bulk sorts)

1 million/mL

(plate sorts)

Threshold Rate 18 – 22 x 105 cells/sec ~ 8,000 events/sec ~6,000 events/sec ~3,000 events/sec ~500 – 1000

Events/sec

Optimal Injection speed*** 20-35 µL/min

 

 

Sample Injection and Collection Set-Up
Set-Up Tubes Sorting Pools Operating Temperature
Injection 12 x 75 mm  (5mL)

15 (mL) conical

Room Temperature

4 degrees

24 degrees

37 degrees

Collection Eppendorf tubes

12 x 75 mm  (5mL)

4 – way sorting Collection device is attached to variable temperature controlled water bath.
15 mL conical 2 – way sorting
Multi-well plates

(96, 48, 24, 6)

Glass slides

Chamber slides

1-way sorting Sorting into multi-well plates is performed at room temperature.

 

Notes:

 

* Large delicate cells may not always do well when sorted on the Aria. If cells are extremely fragile, consider speaking with an operator about sorting your samples with the BD Influx.

**Cell Concentration – values given are a maximum concentration – concentrations higher than this will require dilution and increased sorting time.

*** Injection speed is dependent on actual cell concentration.  At 30 µL/min the instrument will finish 1mL of sample in 35 minutes.  If samples are too concentrated, the injection speed may be decreased or the sample may be diluted to achieve good sorting efficiency.

 

Download a copy of the FACS Aria IIu’s specifications – ARIA_Specs

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