Rate & Services



Operator Assisted Cell Sorting

  • Cell sorting on our FACS Aria IIIu or Influx requires the skill of a trained operator to acquire, analyse and eventually sort cells based on client directives.
  • Sorting is performed under sterile conditions in Class IIA biosafety cabinets.
Operator Assisted Data Acquisition and Analysis
  • Clients who have not been trained to use the analysis systems or who are interested in achieving quick and reliable results may request professional service to acquire and analyze their data.  Once acquisition is complete, operators explain results and also provide data summaries which may include publication ready images (if requested).
Software Tutorials
  • To better understand your data and its analysis our staff can teach you to use popular data analysis platforms so you can create your own publication ready figures and statistics for your data sets.
FACS Melody Unassisted- Cell Sorting:
  • Regular clients of the facility that have been trained by us to use our analyzers can receive training to sort their own samples on the BD FACS Melody.
  • Qualifications will be assessed by staff prior to agreeing to the training.
Instrument Training for New Users
Variable – from $550 -750 Depending on experience
Lab Orientation and Training for Experienced Users
  • Analyzers only
Unassisted Acquisition:
     BD FACS Canto II /BD LSRII Systems
     BD LSR Fortessa- 4- laser System
     BD LSR Fortessa X20 – 5-laser Systems
     BD High Throughput Samplers


Non-Academic Services

  • Operator-assisted and unassisted usage rates can be provided upon request for companies or non-profit groups wishing to use the facility



Analysis Software:

  • FlowJo

    Copies may be purchased for laboratories internal to the University  of Toronto at a discounted rate (~375 CAD/license/year)

    • Licences are charged annually from June to the following May each year to the Principle Investigator’s account.
    • Available only to labs directly financially linked through U of T.
    • Contact the facility to initiate the registration process via email.  Please include the hardware/mac address for your system in the message.
    • Follow these instructions to locate the hardware address for your workstation.
      • Windows:

        • Run the cmd function and enter  “ipconfig/all” in the dos window.
      • MACs:

        • System Preferences dialog box located under the apple icon.
        • Once you have opened the system preferences window, click on “Network”.
        • Choose the type of connection you are using (eg. Ethernet) and DOUBLE CLICK on it.
        • Please ensure to give me the Ethernet ID.
  • For our users do not wish to purchase software licences, a no-cost stand alone workstation is available in the FCF.
    • The workstation is available on a first come first serve basis.

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