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Temerty Faculty of Medicine Flow Cytometry Facility
 
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  • Home
  • Intro to Flow
  • Cell Sorting
    • Influx
    • FACS Aria IIIu
    • BD FACS Melody
    • Sample Prep for Sorting
  • Equipment & Services
    • Instruments
    • Rate & Services
  • Applications
    • Apoptosis
    • Cell Viability
      • Viability Stains
    • Cellular Proliferation and Tracking
    • DNA Content & Ploidy Analysis
      • Cell Cycle Dyes
  • Protocols
  • Advanced Concepts
    • Setting Instrument Voltages
  • Policies
    • General Rules on Instrument Care
    • Book My Lab – Usage Instructions
  • Contact
  • Biosafety
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  • Message Board

Protocols

Cell Sorting
  • Buffers for Cell Sorting and Sorting Sample Preparation
  • FACS Aria Specifications
  • BD Influx Specfications
Immunophenotyping
  • Direct Immunofluorescent Staining
  • Intracellular Staining
Cell Cycle Analysis
  • DAPI Cell Cycle Analysis with Alcohol Fixation
  • DAPI Cell Cycle with PFA Fixation for Combination with Surface Stains
  • PI Cell Cycle with Alcohol Fixation
  • Live Cell Immunofluorescent Staning with Cell Cycle Analysis
  • Surface Staining with fixed Cell Cycle Analysis (H33342)
  • Flow Cytomtery of Yeast DNA
Cell Viability/Dead Cell Discrimination
  • Dead Cell Discrimination for Live/Unfixed Cell Analysis
  • Dead Cells in Paraformaldehyde Fixed Samples
Proliferation
  • Cellular Proliferation with CFSE
Fixing Cells
  • Fixing Cells with Paraformaldehyde

 

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  • Home
  • Intro to Flow
  • Equipment & Services
  • Instruments
  • Cell Sorting
  • Biosafety
  • Applications
  • Protocols
  • Advanced Concepts
  • Policies
  • Resources
  • Message Board
  • Contact
  • DNA Content & Ploidy Analysis
  • Book My Lab – Usage Instructions
7226-1 King's College Circle
Medical Sciences Building
University of Toronto
416-978-6524
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